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chibi_trillianRandomness, found when I was supposed to be working on a grant proposal.
Which Bleach Character Are You?
Cost of one mitochondrial DNA extraction kit, good for 50 assays: $365.
Cost of mtDNA probes: ~$300.
Cost of compensating sick people for a chunk of muscle out of their leg: Depends, how much would you want to be paid for an invasive procedure like that?
Cost of using campus-available equipment: Thankfully free.
Finally getting your fucking grant proposal budget straightened out the night before it's due: Priceless.
^.^;
Now who the hell am I gonna ask for money?
Which Bleach Character Are You?
Cost of one mitochondrial DNA extraction kit, good for 50 assays: $365.
Cost of mtDNA probes: ~$300.
Cost of compensating sick people for a chunk of muscle out of their leg: Depends, how much would you want to be paid for an invasive procedure like that?
Cost of using campus-available equipment: Thankfully free.
Finally getting your fucking grant proposal budget straightened out the night before it's due: Priceless.
^.^;
Shite I have to do for my grant proposal experiment:
- Get approval from my institution’s review board!!!!!!!! for human experimentation
- Do the informed consent boogie
- Collect KSS subjects—as large group as possible, say n=50?
- Need:
- Age of onset
- Severity of course
- Size of mtDNA deletion, if known
- Have muscle punch biopsies conducted at
- Compensate subjects
- Homogenize samples
- Run through my spiff-ass mtDNA isolation kit thingie that cost me e-fuckin-nough to pay my rent
- PCR time!
- Run gels! Wee!
- Southern blot time! Hardcore!
- Experiment based solely on size of deletion, or on location too? Just size, for now, easier/cheaper to determine
- Monitor subjects for severity of disease
- Hope funding doesn’t dry up
Now who the hell am I gonna ask for money?
